Complement receptor 3 (CR3) mediates both opsonic and nonopsonic phagocytosis of bacteria. Leukocyte adhesion deficiency (LAD) allows for the study of CR3-dependent phagocyte-bacterial ingestion, since LAD phagocytes do not express this receptor. Phagocytes from an infant with LAD were unable to ingest 50% of the Pseudomonas aeruginosa strains studied, which indicates a requirement for CR3. However, the remaining strains were phagocytosed in the absence of CR3, and ingestion was blocked by monoclonal antibodies directed at CD14. This CR3/CD14 receptor bias was further confirmed by using thioglycollate-elicited murine peritoneal macrophages, which have nonfunctional CR3 before activation. Results indicate that either CR3 or CD14 is involved independently in nonopsonic phagocytosis of different P. aeruginosa strains. Clearance of P. aeruginosa from the endobronchial space may be facilitated by nonopsonic phagocytosis, since low levels of opsonins are present. The impact of lung infection with P. aeruginosa may be determined, in part, by the phagocytic receptor that mediates ingestion.

Original publication

DOI

10.1086/319685

Type

Journal article

Journal

J Infect Dis

Publication Date

15/04/2001

Volume

183

Pages

1214 - 1220

Keywords

Adult, Antibodies, Monoclonal, Antigens, CD, Antigens, CD14, Cystic Fibrosis, Fetal Blood, Flow Cytometry, Humans, Infant, Newborn, Leukocyte-Adhesion Deficiency Syndrome, Macrophage-1 Antigen, Macrophages, Monocytes, Neutrophils, Phagocytosis, Pseudomonas aeruginosa, Reference Values, Species Specificity