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<jats:sec><jats:title>Summary blurb</jats:title><jats:p>m<jats:sup>6</jats:sup>A-modification in the 5’ vicinity of the coding sequence of transcripts provides a selective mechanism for triaging mRNAs to stress granules and is mediated by YTHDF3 ‘reader’ protein.</jats:p></jats:sec><jats:sec><jats:title>Abstract</jats:title><jats:p>Reversible post-transcriptional modifications on messenger RNA emerge as prevalent phenomena in RNA metabolism. The most abundant among them is N<jats:sup>6</jats:sup>-methyladenosine (m<jats:sup>6</jats:sup>A) which is pivotal for RNA metabolism and function, its role in stress response remains elusive. We have discovered that in response to oxidative stress, transcripts are additionally m<jats:sup>6</jats:sup>A-modified in their 5’ vicinity. Distinct from that of the translationally-active mRNAs, this methylation pattern provides a selective mechanism for triaging mRNAs from the translatable pool to stress-induced stress granules. These stress-induced newly methylated sites are selectively recognized by the YTH domain family 3 (YTHDF3) ‘reader’ protein, thereby revealing a new role for YTHDF3 in shaping the selectivity of stress response. Our findings describe a previously unappreciated function for RNA m<jats:sup>6</jats:sup>A modification in the oxidative-stress response and expand the breadth of physiological roles of m<jats:sup>6</jats:sup>A.</jats:p></jats:sec>

Original publication

DOI

10.1101/357012

Type

Journal article

Publisher

Cold Spring Harbor Laboratory

Publication Date

27/06/2018