Systems Vaccinology/Systems Biology
Our aim is to understand disease pathogenesis and host responses to infection and vaccination.
Systems biology is a rapidly expanding research discipline aiming to integrate multifaceted datasets generated using state-of-the-art high- throughput technologies such as arrays and next-generation sequencing. Combined with sophisticated computational analysis we are able to interrogate host responses to infections and vaccination on a systems level, thus generating important new hypotheses and discovering unknown associations between immunological parameters.
Systems biology of enteric fever
We are aiming to delineate the host responses to enteric fever by using large datasets consisting of proteomics, antigen arrays, metabolomics and transcriptomics derived from the human challenge model. A major aim of this program is to identify correlates of protection following vaccination and challenge. To complement data from humans, the research program also consists of in vitro and in vivo animal studies.
Utilising the systems toolkit to elucidate the mechanisms underlying responses to infant immunisation (lead: Daniel O'Connor)
Vaccine responses and the persistence of vaccine-immunity vary considerably between individuals, this is particularly relevant in childhood, as infants generally have lower magnitude immune responses that wane more rapidly than adults. Our lab is utilising contemporary methods, such as genome-wide genotyping and next-generation sequencing, to explore the mechanisms underlying immunological and physiological responses to childhood vaccinations.
B-CELL RECEPTOR REPERTOIRE (LEAD: DOMINIC KELLY)
B cells are an essential aspect of the adaptive immune system and produce antibodies in response to the numerous pathogens encountered throughout an individual’s lifetime either through infections or vaccination strategies. Each B cell has a unique immunoglobulin transmembrane receptor that is able to bind the antigen prior to secreting antibody. Our group uses next generation sequencing technologies to provide in depth knowledge of the diversity of the repertoire . There is potential for this technology to be applied in a variety of different applications including; the assessment of vaccination strategies, disease diagnostics, and aid therapeutic antibody discovery.
-Nepal Diagnostics Study
-Vaccines Against Salmonella Typhi (VAST)
-Paratyphoid challenge study (P1)
-EU Childhood Life-threatening Infectious Diseases Study (EUCLIDS)
-Personalised Risk assessment in febrile illness to Optimise Real-life Management across the European Union (PERFORM)
We collaborate with:
Wellcome Trust Sanger Institute, Cambridge.
Nationwide Hospital, Ohio State University, USA.
EMBL Australia, SAHMRI, Oz.
Computational Systems Biology Laboratory, University of Sao Paulo.
Wellcome Trust Centre for Human Genetics, Oxford, UK.
Genome Institute of Singapore, Singapore
Oxford Gene Technology, Oxford, UK
Imperial College London, London, UK